鼻咽癌组织中EBV-BARF0基因序列的测定

Sequence Analysis of EBV-BARFO Gene in Nasopharyngeal Carcinoma from Guangdong Area

目的:EB病毒EpsteinBarrvirusEBV存在的变异影响到其生物学功能(如LMP1基因)。BamHIA区右向框0(BamHIArightwardfram0BARF0)是在鼻咽癌NasopharyngealCarcinomaNPC病人中检出率很高的一个EB病毒基因,对其变异性的研究尚未见报道,本研究是为了明确广东鼻咽癌组织中EBVBARF0基因的序列及其变异。方法:应用PCR技术从20例鼻咽癌组织中,扩增EBV基因BARF0,并对它们的序列进行测定。结果:与标准株B958相比,鼻咽癌组织中EBVBARF0均有4个位点发生突变:160473(G→T)、160545(C→T)、160701(C→A)、160707(G→C),并导致相应的氨基酸的改变:2(Ala→Ser)、26(Leu→Phe)、78(Arg→Ser)、80(Ala→Pro)。结论:由于EBV的BARF0基因在鼻咽癌细胞株及活检组织中100%表达,在淋巴瘤组织及淋巴瘤细胞株中表达较低或不表达,本研究首次报道鼻咽癌组织中EBVBARF0的序列分析,与B958相比存在变异,并导致了蛋白质的一级结构的改变,推测该基因很可能在鼻咽癌变过程中起着重要的作用。

Objective:There were variations of EBV genome f rom different areas and they may affe ct the biologic function of EBV(such as LMP1).EBV-BARF0was highly detected in th e patients with nasopharyngeal carc inoma (NPC)and its variations have not been rep orted.This study was designed to determine the sequence and variation of EBV-BARF0gene in the patieats with NPC from Guangdong area.Methods :PCR was used to amplify the EBV-BARF0gene in 20patients with NPC and the production s were sequenced on the ABI377.Results:Comparing with standard B95-8,there were four loci with variances i ncluding:160473(G→T),160545(C→T),160701(C→A),160707(G→C)of sequences and 2(Ala→Ser),26(Leu→Phe),78(Arg→Ser),80(Ala→Pro)of amino acid in 20patients with NPC.Conclusion:Since the BARF0gene of EBV is expressed in all nasopharyngeal carcinoma c ell line and biopsies,but it is not expressed or less frequenly in lymphoma tissues and cell lines,the authors firstly reported the variation of EBV-BARF0which compared with B95-8in the patieats with NPC from Guangdong area.These su ggest that the gene may play an important role in the carcinogenesis and development of nasopharyngeal carcinoma .