大肠癌患者血清癌胚抗原、可溶性Fas、白细胞介素-6水平测定及其临床意义

Determination of Soluble Fas,Carcinoembryonic Antigen,and Interleukin-6in Serum of Patients with Colorectal Carcinoma and Clinical Significance

目的:探讨大肠癌患者血清癌胚抗原(carcinoembryonicantigenCEA)、可溶性FassolubleFassFas、白细胞介素-6interleukin6IL6水平的临床意义。方法:采用双抗体夹心ELISA方法检测162例大肠癌患者术前、术后血清中sFas、IL6表达水平,同时测定了相关免疫指标及血清CEA水平。结果:大肠癌患者血清sFas水平为(20.97±8.19)ng/L,阳性率为53.70%;IL6水平为(36.87±11.20)ng/L,阳性率67.91%;CEA水平为(32.52±10.81)μg/L,阳性率41.98%三者均非常显著高于良性病变及对照组。大肠癌患者术前血清CEA、sFas、IL6水平均显著高于术后(P<0.01以sFas、IL6水平升高更明显。血清sFas水平与癌转移相关,而血清IL6与肿瘤大小及癌转移明显相关(P<0.01。仅血清sFas水平与淋巴细胞转化率、LAK及NK活性呈显著负相关。CEA、IL6、sFas三者之间均呈显著正相关。结论:大肠癌患者血清sFas、IL6检测阳性率高于CEA。sFas、IL6反映了肿瘤的增殖和转移情况,这可能与sFas抑制了免疫功能和肿瘤细胞的凋亡有关;IL6有促进肠癌细胞的增殖作用。三者联合检测对大肠癌患者的临床诊断、判断预后及监测疗效有重要作用。

Objective:This study was designed to determine the serum carcinoembryonic antigen(CEA),soluble Fas(sFas),and interleukin-6(IL-6)levels in colorectal cancer and analyze their clinical significance.Methods :Serum sFas and IL-6were tested in pre-operated and post-operated 162patients with colorectal cancer using double anti body sandwich ELISA method,serum CEA and immune indicators were also examined.Results:Levels of serum CEA,sFas and IL-6[(32.52±10.81)μg /L,(20.97±8.19)ng /L,(36.87±11.20)ng /L,respectively]in the colorectal cancer patients with the positive ra te(sFas:53.7%;IL-6:67.91%;CEA:41.98%)were significantly higher than those in healthy control group and benign patients(P<0.01),the post-operated patients showed a lower levels CEA,sFas,and IL-6than the pre-operation patient s(P<0.01),serum levels of sFas and IL-6in post-operated patients were the lowest.Serum sFas level was correlated with tumor metastases,serum concentration of IL-6was correlated strongly with tumor size and metastases(P<0.01).Serum concentration of sFas was correlated negatively with lymphocyte transformated rate,NK or LAK killing rate,and had marked co rrelation among CEA,IL-6,and sFas.Conclusion:The positive rates of serum sFas and IL-6in the colorectal carcinoma patients were higher than those of th e serum CEA.Serum sFas and IL-6levels reflected indirectly situation of p roliferation and metastases in tumo rs.sFas may inhibit the patientsim mune function and tumor cells apoptosis ,and IL-6m ay accelerate proliferation of the c olorectal carcinoma cells .These re sults indicate that combinative measurin g of the serum levels of CEA,sFas,and IL-6may be an useful method for clini cal diagnosis,disease prognosis,and t reatment effect in colorectal patients.