核糖体蛋白L26基因的克隆及其在肝癌中的表达

Cloning and expression of ribosomal protein L26 in hepatocellular carcinoma

目的 克隆和分析与肝细胞癌发生发展相关的基因。方法 应用差异显示技术获得2 4例肝癌及其癌旁组织表达差异的目标基因片段 ,对其进行克隆、测序 ,并在基因数据库中检索进行同源性比较 ,应用印迹法 (Northern)杂交技术研究其正常组织分布和肝癌中表达的变化。结果 肝癌组织中获得一目标基因片段经测序证明其来源于一已知基因核糖体蛋白L2 6(RPL2 6) ,North ern杂交分析表明该基因在各种组织中广泛表达 ,83 .3 %原发性肝癌患者癌组织中RPL2 6表达显著高于癌旁组织。结论 RLP2

Objective To clone and analyze hepatocellular carcinoma (HCC) associated gene. Methods Differential display by polymerase chain reaction(DD PCR) strategy was used for isolation of altered expression genes in 24 cases of HCC and paracarcinoma tissues. The PCR clones were then sequenced and checked for significant alignments in GenBank. The distribution in normal tissues and expression in HCC were examined by Northern blot analysis. Results A cDNA fragment which had 100% homology with ribosomal protein L26 (RPL26) was isolated from HCC tissue. Northern blot analysis showed that this gene was widely distributed in normal tissues. There were 83.3% patients with higher level of RPL26 in HCC tissues than in paracarcinoma tissues. Conclusion RPL26 might serve as a valuably biological marker in HCC development.