乙型肝炎病毒x基因上调肝癌细胞胰岛素样生长因子-Ⅱ胚胎启动子活性的研究

Up-regulation of the activities of insulin-like growth facfor-Ⅱfetal promoters by hepatitis virus x gene in hepatocellular carcinoma cell line

目的 研究乙型肝炎病毒x基因 (HBx)对肝癌细胞胰岛素样生长因子 Ⅱ (IGF Ⅱ )启动子活性的影响 ,探讨HBx基因影响肝癌恶性表型的分子机制。方法 应用定量逆转录 聚合酶链反应 (RT PCR)法检测QGY770 1肝癌细胞株和转HBx基因肝癌细胞株QGY/HBx的IGF Ⅱ成年启动子P1与胚胎启动子P3、P4的活性。结果 与QGY770 1细胞相比 ,QGY/HBx细胞IGF Ⅱ基因P1启动子活性无明显改变 (分别为 3 .12± 1.18与 3 .74± 1.87,P >0 .0 5 ) ,而P3与P4活性显著升高 (2 .2 7± 0 .68、3 .97± 1.66与 1.2 0± 0 .3 9、1.45± 0 .43比较 ,P <0 .0 5 )。结论 HBx基因可上调肝癌细胞IGF Ⅱ基因胚胎启动子P3、P4活性 。

Objective To study the effects of hepatitis virus x gene (HBx gene) on the activities of insulin like growth facfor Ⅱ(IGF Ⅱ)promoters in hepatocellular carcinoma (HCC) cell line for investigation of the molecular mechanisms of HBx gene affecting the malignant behavior of HCC cells. Methods By means of quantitative reverse transcription polymerase chain reaction(RT PCR), the activities of adult promoter P1 and fetal promoters P3 and P4 of IGF Ⅱgene were determined in QGY7701 HCC cell line and QGY/HBx HCC cell line with HBx gene modified. Results Comparing with QGY7701 cells, the promoter P1 activity of IGF Ⅱ did not obviously change (3.12±1.18 vs 3.74±1.87, P >0.05), but the P3 and P4 activities were increased significantly in QGY/HBx cells (2.27±0.68, 3.97±1.66 vs 1.20±0.39, 1.45±0.43, P < 0.05 ). Conclusion The HBx gene could up regulate the activities of the fetal promoters P3 and P4 of IGF Ⅱ, which might be a likable important mechanism of HBx gene to enhance the malignant behavior of HCC cells.