骨骼肌卫星细胞梗死心肌移植的细胞因子分泌及血管再生作用

The cell factors secretion and vascular regeneration by the skeletal muscle satellite cell implanted into infarction myocardium

目的 研究骨骼肌卫星细胞梗死心肌移植的胰岛素样生长因子 (IGF) 1、碱性纤维母细胞生长因子 (bFGF)分泌及血管再生的作用。方法 骨骼肌卫星细胞经结扎的冠状动脉左前降支远端灌注移植入梗死区 ,2、4、8周后取标本 ,应用免疫组化学方法检测细胞因子表达及梗死区血管密度。结果 骨骼肌卫星细胞移植 2、4、8周后梗死区IGF 1和bFGF表达分别为 81.68± 3 .3 4、96.87± 7.78、90 .43± 7.3 6及 81.87± 3 .5 8、65 .66± 4.5 7、74.2 0± 6.41,明显高于对照组 (P <0 .0 1) ;同时 ,移植 2、4、8周后梗死区血管密度高于对照组 (P <0 .0 5 )。结论 骨骼肌卫星细胞梗死心肌移植除有心肌再生外 。

Objective To study the insulin like growth factor 1 (IGF 1), basic fibroblast growth factor (bFGF) secretion and vascular regeneration by the skeletal muscle satellite cells implanted into infarction myocardium. Methods Skeletal muscle satellite cells were implanted into the acute myocardial infarct site via the ligated left anterior descending (LAD) artery. Specimens were harvested at 2, 4, 8 weeks after implantation for the expression of cell factor and the density of vascular examination by immunohistiological method. Results The expression of IGF 1, bFGF were 81.68±3.34, 96.87± 7.78 , 90.43±7.36 and 81.87±3.58, 65.66±4.57, 74.20±6.41 respectively in the implanted group at 2nd, 4th, 8th week after transplantation, higher than that in the control group ( P <0.01). At the same time, the vascular density respectively in the skeletal muscle satellite cell implant group, significantly higher than that in the control group ( P <0.05). Conclusion The skeletal muscle satellite cells, after being implanted into the acute myocardial infarction, not only showed myocardial regeneration, but also showed the ability to secrete the cell factors and play an positive effect on infarction myocardium.