雌二醇对大鼠睾丸组织生长发育的影响

Effect of estradiol benzoate on the growth and development of rat testis

目的 研究雌二醇对睾丸组织生长发育的影响及作用机理。方法 以雄性幼年SD大鼠为对象 ,设实验组和对照组 ,实验组大鼠皮下注射苯甲酸雌二醇 (E2 B ,0 .0 4mg/g体重 ) ,于注药后第 14、2 8、42、5 6天取材。行睾丸重量测定、组织病理学观察和凝集素 (ConA ,GS I ,PNA ,SBA和DBA)组织化学实验。结果 实验组睾丸重量 (1× 10 -2 g) 14d为 3 .3 1± 0 .47,2 8d为 4.45±0 .64 ,42d为 6.3 8± 0 .5 5 ,5 6d为 6.41± 0 .81,均明显小于对照组 (14 .47± 1.2 0、2 1.95± 7.72、95 .43± 1.62、10 7.76± 1.2 4,P <0 .0 1) ;与对照组相比 ,实验组睾丸生长发育停滞并出现明显病理学改变 ,对凝集素的反应减弱。

Objective To study the effects of estrogens on the growth and development of rat testis and the mechanism. Methods SD infant male rats were divided into control group and experimental group. In the experimental group, the rats received subcutaneous injection of estradiol benzoate (0.04 mg/g body weight) ant the specimens were harvested at the day 14,28,42 and 56 after administration. The weights of the testis were measured, the morphological changes of the testis were observed and the tissue was stained with 5 biotinylated lectins (ConA, GS I, PNA, SBA and DBA) for light microscopy. Results In the experimental group, the weights (1×10 -2 g) of the rats' testis was 3.31±0.47 at the day 14, 4.45±0.64 at the day 28, 6.38±0.55 at the day 42 and 6.41±0.81 at the day 56, all significantly lighter than in the control group (14.47±1.20, 21.95±7.72, 95.43±1.62, 107.76± 1.24 , respectively, P <0.01). As compared with control group, the growth and development of the testis in the experimental group were retarded and obvious pathological changes occurred, and its response to all the lectins was decreased in the testis. Conclusion Estradiol benzoate could obviously inhibit the growth and development of rat testis.