肝癌患者A-LAK细胞与苯乙酸协同抗瘤的观察

The Synergistic Anti-Tumor Activity of Phenylacetate and Adherent LAK Cells from Patients with Hepatocellular Carcinoma

目的 :简化粘附性LAK(A LAK)细胞的制备方法并观察其与苯乙酸 (PA)的协同抗瘤作用。方法 :采用苯丙氨酸甲酯(PME)处理肝癌患者外周血单个核细胞 (PBMC)而制备A LAK细胞 ;观察人肝癌SMMC772 1细胞株经PA处理后增殖能力的变化 ;并用经PA预处理的SMMC772 1培养上清液作用于A LAK细胞 ,观察A LAK增殖能力与杀伤活性的变化。结果 :采用PME制备的A LAK细胞 ,其增殖能力显著高于非粘附性LAK细胞 (NA LAK)和常规LAK细胞 ;肿瘤细胞经PA作用后 ,生长明显受到抑制 ;肿瘤细胞的培养上清液可明显抑制A LAK的增殖与杀伤活性 ,而经苯乙酸预处理的上清液则抑制作用减弱。结论 :采用PME可简便快速地制备A LAK ,而苯乙酸可与A LAK协同发挥抗瘤作用。

Objective: To improve the preparation of adherent lymphokine-activated killer cells (A-LAK cells) and to study the synergistic anti-tumor effect of phenylacetate(PA) and A-LAK cells. Methods: A-LAK cells were obtained from peripheral blood mononuclear cells (PBMC) of patients with hepatocellular carcinoma (HCC) by using L-phenylalanine methyl ester (PME) to deplete immunosuppressive monocytes. The proliferation of SMMC7721 cell line treated with PA was studied. A-LAK cells were treated with the supernatant derived from SMMC7721 cells treated with PA previously and the changes of proliferation and anti-tumor activity of A-LAK cells were observed. Results: The expansion of A-LAK cells was significantly higher than that of non-adherent LAK (NA-LAK) as well as standard LAK cells. The growth of SMMC7721 cells was significantly suppressed by PA. The supernatant of cultured tumor cells intensely suppressed the proliferation and cytotoxic activity of A-LAK cells, but the suppressive effect of supernatant treated with PA previously was decreased. Conclusion: A-LAK could be simply prepared by using PME, and have the synergistic anti-tumor effect with phenylacetate.