重组鸡痘病毒与核酸疫苗联合免疫提高机体免疫功能研究

Consecutive Immunization with Recombinant Fowlpox Virus and Nucleic Acid Vaccine Plasmid

目的 :探索联合免疫对小鼠细胞免疫和体液免疫功能的影响。方法 :以表达中国流行株HIV 1核心蛋白Gag的重组鸡痘病毒vUTALG作初始免疫 ,以表达相同抗原的核酸疫苗质粒pcDNAG加强免疫 ,对被免疫鼠的细胞免疫和体液免疫功能进行初步测定。结果 :以重组鸡痘病毒首次免疫的联合免疫组与单纯用重组病毒免疫组比较 ,CD4+ T细胞数升高 ,ConA和LPS诱导的T细胞增殖能力均明显增强 ,并能诱导HIV 1特异的血清抗体反应。结论 :以重组鸡痘病毒首次免疫的联合免疫能有效的提高T ,B细胞介导的细胞免疫和体液免疫水平。

Objective: To research influence of consecutive Immunization on cellular and humoral immunity in mice. Methods: We evaluated a consecutive immunization strategy priming with recombinant fowlpox virus vUTALG and boosting with nucleic acid vaccine plasmid pcDNAG encoding HIV-1 capsid protein Gag. Results: In immunized mice, the number of CD4 + cells from splenic lymphocytes increased singnificantly and the proliferation response of splenocytes to Con A, LPS elevated markedly and HIV-1-specific antibody response could be induced. Conclusion: Consecutive immunization could increase cellular and humoral immunity response in mice.