银杏叶中黄酮类成分的研究

Studies on the constituentes of flavonoids from Ginkgo biloba leaves

目的 分离鉴定银杏叶中的黄酮类成分。方法 95 %乙醇提取 ,硅胶及SephadexLH 2 0柱层析分离 ,用UV ,IR ,NMR和MS等方法确定结构。结果 分得 7个黄酮类成分 ,分别为槲皮素 (quercetin ,Ⅰ )、山萘酚 (kaempferol,Ⅱ )、异鼠李素 (isorham netin ,Ⅲ )、芫花素 (ginkwanin ,Ⅳ )、金松双黄酮 (sciadopitysin ,Ⅴ )、银杏素 (ginkgetin -Ⅵ )、和异银杏素 (isoginkgetin ,Ⅶ ) ,以及 7个其它类型成分 ,分别为三十烷酸 (triacontanoicacid ,Ⅷ )、二十八醇 (octacosanol,Ⅸ )、白果醇 (ginnol,Ⅹ )、二十九烷 (nonacosane ,Ⅺ )、β谷甾醇 ( β sitosterol,Ⅻ )、棕榈酮 (palmitone ,ⅩⅢ )和胡萝卜苷 (daucosterol,ⅩⅣ )。 结论 其中化合物Ⅳ ,Ⅷ ,Ⅸ ,Ⅺ ,ⅩⅢ 。

OBJECTIVE To isolated and elucidate the flavonoids from the leaves of Ginkgo biloba L.METHODS The chromatography on silica gel and Sephadex LH-20 was used.The structures were determined by UV,IR, 1H NMR, 13 C NMR and MS spectral analysis.RESULTS Seven flavonoids and seven other compounds were isolated and elucidated as following:quercetin,kaempferol,isorhamnetin,ginkwanin,sciadopitysin,ginkgetin,isoginkgetin,triacontanoic acid,octacosanol,ginnol,nonacosane,β sitosterol,palmitone and daucosterol.CONCLUSION Ginkwanin,triacontanoic acid,octacosanol,nonacosane,palmitone and daucosterol were isolated from the leaves of G.biloba for the first time.