古尼拟青霉对大鼠急性缺血性脑损伤的保护作用及机制研究

Protective effects of paecilomyces gunnii on acute cerebral ischemia injury in rats and its mechanisms

目的 研究古尼拟青霉对大鼠急性缺血性脑损伤是否具有保护作用 ,并探讨其相关机制。方法 采用 4 动脉阻断法 ( 4 VO)复制大鼠全脑急性缺血 30min/再灌注 30min模型。大鼠随机分为假手术组、模型组、古尼拟青霉高、低剂量组和复方丹参组。于造模前连续ig给药 3周 ,再灌注 30min后处死大鼠测定脑组织多项生化指标和观察脑海马CA1区锥体细胞超微病变。结果 与模型组比较 ,古尼拟青霉高、低剂量组均可增加大鼠脑缺血 /再灌注大鼠脑组织中肌酸激酶 (CK)和超氧化物歧化酶 (SOD)的活性 ,降低脑组织中丙二醛 (MDA)的含量 ;高剂量组还能增加脑组织中谷胱甘肽过氧化物酶 (GSH Px)的活性、降低脑组织水及Ca2 +含量 ,并显著改善海马CA1区锥体细胞的超微病变。结论 古尼拟青霉对大鼠急性全脑缺血 /再灌注损伤有一定保护作用 ,其机制可能与提高脑组织中抗氧化酶活性、减轻脑组织生物膜脂质过氧化损伤及拮抗脑细胞内Ca2 +超载有关。

OBJECTIVE To study the protective effects of paecilomyces gunnii(Pg) on acute cerebral ischemia injury in Wistar rats,and discuss its relevant mechanisms.METHOD The model of cerebral ischemia 30 min/reperfusion 30 min induced by four vessel occlusion(4 VO) in rats was used.The rats were randomly divided into sham operation group, model group,high and low groups of Pg and salvia miltiorrhiza group,and were constantly medicated the corresponding drug by PO for three weeks before duplicating model.RESULTS Compared with the model group, Pg high and low groups increased the CK and SOD activities in brain tissue at 30 min reperfusion after 30 min ischemia in rats,and decreased MDA content.Pg high group also increased GSH Px activity and reduced brain water content and Ca 2+ content in brain tissue,and improved the serious pathological process of pyramidal cells in hippocampus CA1 sector as well.CONCLUSION It was concluded that Pg,to a certain degree,was effective on acute cerebral ischemia/ reperfusion injury in rats. The relevant mechanisms may be related to increasing antioxidase activities in brain tissue,decreasing lipid peroxidative damage to biomembrane of brain tissue, and reducing Ca 2+ overload in brain cells.