氦氖激光辐照对孔雀鱼抗病力及生长的影响

THE INFLUENCE OF He-Ne LASER IRRADIATION ON DISEASE RESISTANCE AND GROWTH OF GUPPY, POECILIA RETICULATA

于1989年10月—1990年6月间,进行了氦氖激光散焦扩束辐照对孔雀鱼抗病力及生长发育影响的试验。结果表明,经能量密度为2.86J/cm^2激光处理的仔鱼,在车轮虫和水霉、绵霉等病原体侵袭下,其成活率比对照组高126％。在能量密度为5.37J/cm^2激光辐照下,两个月的幼鱼,其α-淀粉酶和β-淀粉酶活性分别比对照组的高36.4％和52.5％;蛋白酶活性无明显变化;脂肪酶活性降低53.5％。在生长方面,90天雄(雌)鱼的体长比对照组增加8.3％(9.7％),体重增加41.7％(39.6％);当激光的辐照剂量为7.52J/cm^2时,其对机体生长发育所产生的生物刺激作用并没有随着相应增强。

The biostimulating effect of low-power laser radiation is a wellknown phenomenon. In order to determine the action of defocusing He-Ne laser irradiation on fishes, a series of experiments were carried out from October 1989 to June 1990. In the study, the parent fishes of guppy, Poecilia reticulata, were taken from Singapore. About two months, they reached sexual maturation and reproduced naturally.The hatched larvae were divided into control and experimental groups randomly. The larvae of the experimental groups were irradiated once every two days and for 25 times altogether. 1. When Trichodina nobillis, Saprolegnia, and Achlya existed in the cultural environment, the survival rate of guppy treated by He-Ne laser at 2.86 J/cm2 was 79.6% after 50 days. But that of the control group was only 35.2% under the same condition.2. The irradiation of He-Ne laser at 5.37 J/cm2 led to the changes of digestive enzymes in 2-month-old guppy. The activity of α-amylase and β-amylase increased by 36.4% and 52.5% respectively, whereas the activity of lipase decreased by 53.5%, and the activity of protease remained at control level. The body length of 3-month-old guppy increased by 8.3% ((?)) or 9.7% ((?)) over the control level, and the body weight increased by 41.7%((?)) or 39.6% ((?)).3. The biostimulating effect of laser light on growth of guppy was not enhanced with the increase of irradiation dosage to 7.52 J/cm2. This may be related to the decrease of the activity of protease.These results demonstrated that He-Ne laser irradiation can enhance disease resistance as well as some metabolic activities and accelerate the growth of guppy at a specific intensity.