薄层扫描法测定降压药复方天麻片中钩藤碱的含量

Thin - layer Chromatographic Scanning Determination Rhynchophylline content in Fufangtianmapian

钩藤系复方天麻片的君药,为保证其质量,建立钩藤碱的含量测定方法。 采用薄层色谱法测定复方天麻片中钩藤碱的含量。以氯仿—丙酮—三乙醇胺(50∶3∶0.5)为展开剂;于紫外光灯(254 nm)下检视。反射法锯齿扫描:λ_s240nm,λ_R370 nm;狭缝1.25mm×1.25mm,线性化参数;SX=3。 通过方法学考察,点样量在0.484～7.744μg范围内呈良好的线性关系,其回归方程为:Y=1769X+3527,r=0.998;平均回收率为100.1％(n=5)。 试验表明,方法可靠,数据准确,易于操作。

Rhynchophylline is the monarch drug in Fufangtianmapian (a Chinese patent medicine) . To guarantee the quality of Fufangtianmapian, a reliable rhynchophylline determination must be set up. Using thin - layer scanning to determine rhynchophylline content in Fufangtianmapian, a mixture of chloroform, acetone, and ammonia water, (50:3 :0.5) was made, examined under ultraviolet lamp(254 nm) ; dual wavelength reflection zigzag scanning was performed: γs240 nm, γR270 nm; slit width : 1 .25 mm×1 .25 mm; linear parameter: SX = 3 . Results: A methodological survey pointed out there is a good linearity between 0.484 ug to 7.744 ug of the tested rhynchophylline; regression equation: Y = 1769X + 3527 , r = 0.998 ; average recovery is 100.1 % (n = 5) . This method is dependable, simple and easy to do, with accurate results.