期刊文献+

蛋白质在膨胀床吸附层析剂的静态和动态吸附性能 被引量:2

Study on Adsorption Performance of BSA in Streamline DEAE and DEAE Sepharose FF Adsorbents
下载PDF
导出
摘要 以牛血清白蛋白 (BSA)为目标蛋白 ,考察膨胀床用离子交换树脂StreamlineDEAE的静态和动态吸附性能 ,并和离子交换树脂DEAESepharoseFF进行比较 ,实验发现两者的静态吸附性能相似 ,而动态吸附性能差别较大。根据动态吸附数据计算出液膜扩散系数和孔内扩散系数。 Adsorption equilibrium and kinetics were studied for the binding of Bovine Serum Albumin to Streamline DEAE and DEAE Sepharose FF.The static adsorption performance in Streamline DEAE was similar to that of in DEAE Sepharose FF,while the dynamic adsorption characteristics were different.A model for describing the dynamic adsorption was presented,and the film diffusion coefficients and pore diffusion coefficients were determined using this model and the dynamic adsorption data.
出处 《化学工程》 EI CAS CSCD 北大核心 2001年第1期37-40,共4页 Chemical Engineering(China)
基金 国家自然科学基金!重点项目 (批准号 :2 9736 180 )
关键词 蛋白质 膨胀床 吸附层析剂 离子交换 扩散系数 牛血清白蛋白 静态 动态 吸附性能 expanded bed adsorption,ion exchange,diffusion coefficient,BSA
  • 相关文献

参考文献1

二级参考文献2

  • 1Chang Y K,Biotech Bioeng,1996年,49卷,204页
  • 2Chang Y K,Biotech Bioeng,1995年,48卷,355页

共引文献10

同被引文献25

  • 1李明愉,曾庆轩,冯长根,李凯,孙伟娜.离子交换纤维对阿魏酸的吸附和解吸研究[J].中国药学杂志,2005,40(1):40-43. 被引量:22
  • 2胡洪波,张雪洪,梅乐和,姚善泾,朱自强.膨胀床离子交换吸附分离纳豆激酶[J].化学工程,2005,33(4):1-4. 被引量:9
  • 3高大海,梅乐和,盛清,许静,林东强,姚善泾.硫酸铵沉淀和层析法分离纯化纳豆激酶的研究[J].高校化学工程学报,2006,20(1):63-67. 被引量:19
  • 4黄俊,梅乐和,钟春龙,邓福华,姚善泾.重组细胞色素P450 BM-3酶的表达条件及初步纯化研究[J].浙江大学学报(农业与生命科学版),2006,32(1):96-100. 被引量:2
  • 5M·霍夫利特,A·斯泰因比谢尔.生物高分子第1卷:木质素、腐殖质和煤[M].北京:化学工业出版社,2004:141-225.
  • 6MUNOZ C,GUILLEN F,MARTINEZ A T,et al. Laccase isoenzymes of Pleurotus eryngii:characterization, catalytic properties, and participation in activation of molecular oxygen and Mn^2+ oxidation[J]. Applied and Environmental Microbiology, 1997,6:2166 - 2174.
  • 7PEREZ - BOADA M, DOYLE W A, RUIZ-DUENAS F J, et al. Expression of Pleurotus eryngii versatile peroxidase in Escherichia coli and optimization of in vitro folding[J]. Enzyme and Microbial Technology. ,2002,30:518 -524.
  • 8LUCILIA CARAMELO,MARIA JESU S MARTINEZ,ANGEL T MARTINEZ. A search for ligninolytic peroxidases in the fungus Pleurotus eryngii involving a-keto-g-thiomethylbutyric acid and lignin model dimers[ J]. American Society for Microbiology,1999,65(3) :916-922.
  • 9HAKUHNEN N,ANDBERG M,KALLIO J,et al. A near atomic resolution structure of a Melanocarpus albomyces laccase[J]. Journal of Structural Biology ,2008,162:39.
  • 10VARELA E ,JESUS MARTINEZ M ,MARTINEZ A T. Martinez. Aryl-alcohol oxidase protein sequence:a comparison with glucose oxidase and other FAD oxidoreductases [ J ]. Biochimica et Biophysica Acta, 2000,1481 ( 1 ) : 202-208.

引证文献2

二级引证文献4

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部