缩氨基硫脲类钯抗癌配合物初步筛选与作用机理的荧光法研究

Fluorescence Method Used for Preliminary Screening and Study on Mechanism of the Interaction of Thiosemicarbazone and Palladium Complexes as Potential Antitumor Agents

新合成了 8-乙酰基 - 4 -甲基 - 7-羟基香豆素缩氨基硫脲、3-乙酰基 - 7-羟基香豆素缩氨基硫脲及其与钯的配合物 ,以溴化乙锭为荧光探针 ,研究了配合物与小牛胸腺 DNA的作用机制 ,初步判定了配合物的抗癌活性 ,计算了与 DNA的结合常数 .

Two new palladium complexes of 8 acetyl 4 methyl umbelliferone thiosemicarbazone and 3 acetyl umbelliferone thiosemicarbazone have been synthesized.The mechanism of the interaction between the complexes and calf thymus DNA has been studied by fluorescence method by using ethidium bromide EthBr as a fluorescence probe.The antitumor activities and the association constants of complexes were estimated.The results of the proposed fluorescence method is in agreement with those of the MTT method.