家兔中性粒细胞防御素的纯化及其对念珠菌抗菌活性的研究

IN VITRO ANTIFUNGAL ACTIVITY OF NEUTROPHIL DEFENSINS AGAINST SOME CANDIDA Spp.

采用聚丙烯酰胺凝胶电泳分离纯化兔中性粒细胞防御素，兔中性粒细胞防御素ＮＰ２具有抗白念珠菌Ｍ１０１２Ｒ，ＮＩＨ Ａ－２０７，乳酒念珠菌，热带念珠菌和高里氏念珠菌等菌的活性，且抗菌活性随防御素浓度的升高而增强．

To investigate the role and mechanism of neutrophil defensins against some Candida spp. Neutrophil defensins were purified by polyacrylam ide gel electrophoresis (SDS－PAGE), and neutrophil defensin NP2 were tested for i ts antifungal activity against Candida albican M1012R, NIH A－207, Candida kefyr , Candida tropicalis and Candida guilliermondii. NP2 was successfully isolated an d purified by AU－PAGE, and demonstrated concentration－dependent killing of C andid albican M1012R, NIH A－207, Candida kefyr, Candida tropicalis and Candida guilliermondii in vitro. Acid urea polyacrylamide gel electrophores is is simple an d convient in isolating and purifying protein and polypeptides, neutrophil defen sin NP2 exerted potent antifungal activity against Candida spp.