高温胁迫对^(14)C-水杨酸在葡萄苗中运转分配的影响

Effects of Elevated Temperature on Transportation and Distribution of ^(14)C-salicylic Acid in Grape Seedlings

用 38℃高温和 2 7℃正常温度分别处理14 C SA标记的葡萄幼苗叶片 1,3,6 ,12h ,发现常温下14 C SA向外运转的很少 ,而高温下向外运转的更少。高温处理和常温对照的14 C SA都既向上运输又向下运输 ,以向下运输为主 ,但无论向上运输还是向下运输 ,高温下要比常温下运输的少一些。在常温下 1,3,6h ,14 C SA以根系分配的最多 ,12h以茎中分配最多 ,而在高温处理下 1,3h以根中分配最多 ,6 ,12h则以茎中分配最多。高温处理和常温对照下都是离引入叶越近的茎段和叶片 (除引入叶 ) ,14 C

All leaves except 4th,5th,6th,7th and 8th ones of grape seedlings were cut off. The sixth leaves labeled with SA were subjected to an elevated temperature of 38℃ or normal temperature 27℃ 1,3,6,12 h while the other parts of seedlings were at 27℃.The results showed that 14 C SA was transported out less at 38℃ than at 27℃(Fig.3).We proposed that SA was the signal of heat stress. 14 C SA was transported either downwards or upwards at 38℃ or at 27℃,primarily downwards,but 14 C SA was transported less both downwards and upwards at 38℃ than 27℃(Fig.4). 14 C SA was distributed mostly in root at 1,3,6 h, mostly in stem at 12 h, but at 38℃, mostly in root at 1 h or at 3 h, mostly in stem at 6 h or at 12 h (Fig.5).The 14 C SA distribution to leaf or stem parts of the same location at 38℃ was different with 27℃, morever, at both 38℃ and 27℃, it was distributed nearly(Figs.6,7).