秋水仙碱对大麦离体培养小孢子存活与成苗的影响

The Effect of Colchicine on Survival and Regeneration of Isolated Microspores in Barley

以 4份大田生长的优良大麦品种 /品系为材料 ,采用超速旋切法分离小孢子进行培养。提取液和预处理液中添加适当浓度的秋水仙碱可明显提高大麦小孢子的存活率。

The effects of colchcine on the survival percentage and on the regeneration frequency were tested in culture microspore from 4 cultivars of barley. The results showed that: CPW solution containing mannitol of 0.3 mol/L and colchicine 10 mg/L used as isolation and pretreatment solution was essential for microspore embryogenesis(Table 3); the survival percentage of isolated microspores could be increased by isolation solution supplemented with colchicine 10 mg/L(Table 1); pretreatment of freshly isolated microspores with CPW solution containing mannitol 0.3 mol/L and colchicine 10 mg/L (25℃, 2 d) could decrease the death rate of microspores after culture for 3 d(Table 2)and increased green plant regeneration(Table 4). According to the comparison, the microspore embryogenesis and green plant regeneration frequency were increased by addition of 10 mg/L of colchicine to isolation and pretreatment solution.