香菇原生质体分离诱变育种研究

THE BREEDING STUDY BY UV-INDUCING PROTOPLASTS OF LENTINUS EDODES

通过ＵＶ处理香菇原生质体后得到的１０５株再生菌株与它们亲本菌株的菌丝生长速度，产量和出菇期进行了比较。约有３０％的再生菌株获得了较高的产量和较早出菇期的优良特性、多次继代培养证明，这些再生菌株获得的优良特性稳定。研究结果表明，香菇原生质体分离诱变是一种很有应用价值的食用菌菌种选育方法。

The experiment was carried out to inquire about the possibility of strain selection and improvement in Lentinus edodes through protoplast formation and mutagenesis by UV. Mycelial growth rate, yield, and period of fructification of 105 selected regenerants from UV-treated protoplasts have been measured and compared with their parents. Several selected regenerants achieved some better qualities such as higher yield and earlier fruiting. Several repeated cultivations of these regenerants reconfirmed their new capacities. The results indicated that protoplast formation and mutagenesis are the potential methods for strain selection and improvement in edible fungi.