摘要
体细胞胚的诱导是大豆体外再生的关键。基因型、诱导光周期、外植体的荚位、蔗糖浓度等因素 ,可导致诱导频率及正常胚比例不同 ,影响植株再生。本研究选用黑龙江省主栽大豆基因型的未成熟子叶 ,在含高浓度生长素的MSB培养基上诱导体细胞胚产生。合丰 2 5和东农 781 9为优选基因型 ;生育前期下部荚位大小为 2～ 4mm未成熟子叶体细胞胚发生效果最好 ;四种光周期下体细胞胚诱导频率相近 ,但连续弱光下正常胚比例高 ;NAA诱导优于 2 - 4,D ;1 0mg/lNAA与1 .
Somatic embryo induction was the key in soybean regeneration. Genetype,inducing photoperiod, explant pod site in and sucrose concentration were the main factors in the somatic embryo induction,which lead to the different embryogenesis efficiency and the number of normal embryos. Cultivars Hefeng25 and Dongnong7819 were desiable genotype.2-4mm long immature cotyledons from the lower part of plant at the early stage of growth and develpment were more efficent for embryogenesis. The embryogenesis frequncy under four photoperiod were similar, but the frequncy of normal embryos was higher under continuous dim light. NAA was superior to 2,4-D in embryogenesis; combination of 10mg/lNAA and 1.5% sucrose was better.
出处
《植物研究》
CAS
CSCD
北大核心
2001年第2期210-214,T001,共6页
Bulletin of Botanical Research
关键词
大豆
体细胞胚
诱导因素
Soybean
Somatic embryos
Induction
