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羊草种群遗传分化的RAPD分析I:扩增片段频率的变化 被引量:9

GENETIC DIFFERENTIATION IN LEYMUS CHINENSIS POPULATIONS REVEALED BY RAPD MARKERS I: VARIATION ON THE FREQUENCIES OF AMPLIFIED FRAGMENTS
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摘要 松嫩草原上分布着灰绿型和黄绿型两种叶色类型的羊草。为进一步明确这两种叶色类型种群分化的分子基础 ,对其 9个种群 1 0 5个个体进行了RAPD分析。 1 5个随机引物 ( 1 0nt)共扩增出 1 49个多态性片段 ,总的多态位点为 96.1 % ;黄绿型与灰绿型种群间在 1 0 .1 %的多态性片段上明显不同 ;相似生境或同一地域的种群在一些位点上表现出相似或相同的变化 ; Leymus chinensis is a rhizome grass species,which has 2 leaf-colored types, a grayish green and a yellowish green, in the Songnen Plain of China. To reveal the molecular bases of genetic differentiation between the 2 types, RAPD was conducted on 105 individuals from 9 populations by using 15 primers. RAPD produced 155 amplified fragments, and total percentage of polymorphic loci is 96.1. 10.1% polymorphic loci were different between the 2 types. There were similarities of certain polymorphic loci among the populations sampled in the similar habitat. There were characterized polymorphic loci for 9 populations respectively.
出处 《植物研究》 CAS CSCD 北大核心 2001年第2期272-277,共6页 Bulletin of Botanical Research
基金 国家教委跨世纪优秀人才计划基金!项目资助
关键词 羊草 遗传分化 RAPD 扩增片断频率 Leymus chinensis genetic differentiation RAPD
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