籼型光敏核不育水稻育性可转换性的基因定位和基因互作研究

The Gene Mapping and Interaction on the Fertility Reversibility in Photoperiod-sensitive Genic Male Sterile Rice (Oryza sativa L)

本研究以来源于农垦 58S的籼型光敏核不育系培矮 64S(短日条件下育性难转换 )和 890 2S(短日条件下育性易转换 )及其F1 、F2 群体为材料 ,通过短日不同光温和不同生态条件 4种处理 ,利用RFLP分子标记研究了影响光敏核不育水稻在短日条件下的育性可转换性的遗传、基因定位和基因互作 ,主要结果表明 :影响光敏核不育水稻的育性可转换性表现为微效基因的作用 ,定位了 7个控制光敏核不育水稻的育性可转换性QTL ,即S2、S3a、S3b、S5、S8和S1 0。揭示了基因互作真实存在于光敏核不育水稻中 ,基因互作形式和互作类型对光敏核不育水稻的育性可转换性的影响表现多种多样 ,不同类型的基因互作所解释的遗传变异处于 2 .1 5%～ 1 0 .0 7%之间。

In this study we selected two photoperiod_sensitive genic male sterile(PSGMS) lines of indica rice derived from Nongken 58S: Peiai64S which is difficult to reverse fertility under short_day condition, and 8902S which is easy to reverse fertility. A cross F 1 and F 2 populations from the cross between Peiai64S and 8902S were used for a molecular marked_based investigation into the inheritance, gene mapping and interaction of PSGMS fertility reversibility under 4 different short_day conditions. The inheritance of fertility reversibility showed quantitative trait performance and 7 QTLs with a complex epistasis interaction detected in PSGMS, they are S2, S3a, S3b, S5, S8 and S10. The gene interaction was really existed and effected the fertility reversibility in PSGMS. The types and kinds of epistasis were showed complexity. The genetic variance explained was between 2.15%～10.07% of fertility reversibility in PSGMS.