摘要
酵母基因中断技术是研究酵母基因功能的重要手段 ,自 80年代初诞生以来经历了不断的改进和发展 .PCR介导的酵母基因中断技术 ,大大简化了操作 ,实现了酵母基因的精确缺失 ;酵母基因的多重中断技术 ,可在酵母内实现多个基因的中断 ;可进行大规模基因中断和功能分析的酵母基因中断技术 ,适应了在酵母全基因组测序完成的情况下进行功能基因组学研究的要求 .酵母基因中断技术对人类基因功能研究也有很大启示作用 .
Gene disruption by homologous recombination is a powerful tool for investigating gene function in yeast. Since 1980's, it has been developed a lot. PCR-mediated gene disruption technique makes the manipulation easier and it can be used to precisely delete genes in yeast. Multi-gene disruption technique can delete several genes successively in the same yeast strain. After the completion of the yeast Saccharomyces cerevisiae genome sequencing, the gene disruption technique for systematic analysis meets the need of the functional genomics in yeast. It also enlighten the study on human functional genomics.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2001年第2期176-180,共5页
Progress In Biochemistry and Biophysics
基金
国家863计划资助项目! (86 3 10 2 11 0 2 0 2 )&&
关键词
酵母
基因中断
功能基因组学
yeast
gene disruption
functional genomics
