听源性遗传癫痫易感大鼠大脑皮层胆囊收缩素mRNA的原位杂交检测(英文)

IN SITU HYBRIDIZATION DETECTION OF CHOLECYSTOKININ mRNA IN CEREBRAL CORTEX OF AUDIOGENIC SEIZURE PRONE RATS

本实验用原位杂交法 ,对听源性遗传癫痫易感大鼠 (P77PMC)癫痫发作前、单次癫痫发作和多次发作时大脑颞皮层CCKmRNA阳性信号进行了检测。结果显示 :(1)单次和多次癫痫发作后颞皮层CCKmRNA阳性神经元数量明显增加 (P <0 .0 1) ;(2 )多次癫痫发作者上述脑区CCKmRNA阳性神经元数量较单次癫痫发作有明显的下降 (P <0 .0 1)。大脑颞皮层CCKmRNA增高表明 ,CCKmRNA在癫痫发作过程中起了某种作用 ;多次癫痫发作大鼠CCKmRNA表达降低提示 ,单次和多次癫痫发作时大脑皮层对CCKmRNA转录的调控可能存在不同的机制。

Using in situ hybridization method,we examined CCK mRNA positive signal in temporal cerebral cortex of audiogenic seizure prone rats(P 77 PMC)before and after a single or multiple seizures.The results were as follows:(1)CCK mRNA levels were elevated significantly in temporal cortex after a single or multiple seizures;(2)CCK mRNA level in multiple consecutive seizure rats was lower than that in a single seizure rats. The increased CCK mRNA positive signal indicates that CCK mRNA in cerebral cortex may play a role in seizure associated processes. The decreased CCK mRNA levels after multiple consecutive seizures may suggest differential regulation of CCK transcription by single and multiple seizures.