低氧预处理提高离体培养的下丘脑细胞缺氧耐受性及其与线粒体膜电位稳定性的关系

Correlation between enhanced anoxic tolerance induced by hypoxic preconditioning and the stability of mitochondrial membrane potential in cultured hypothalamic cells

本文用新生大鼠下丘脑培养细胞 ,研究了低氧预处理对下丘脑细胞缺氧耐受性的影响及其与线粒体膜电位的关系。结果显示 :在急性缺氧条件下 ,低氧预处理可以提高细胞存活率 ,减少乳酸脱氢酶漏出 ;此外 ,低氧预处理可以使线粒体膜电位在缺氧时保持相对高的水平 ,并诱导B淋巴细胞 /白血病 2 (B celllymphoma/leukemia 2 ,bcl 2 )高表达。结果提示 ,低氧预处理能提高下丘脑细胞的缺氧耐受性 ,其机制与线粒体膜电位稳定性增强有关 ;低氧预处理诱发bcl

The relationship between enhanced anoxic tolerance induced by hypoxic preconditioning and mitochondrial membrane potential (MMP) was studied in cultured hypothalamic cells. Dynamic changes in MMP were monitored by confocal laser scanning microscopy and expression of B cell lymphoma/leukemia 2 (bcl 2) was examined by flowcytometry. Hypoxic preconditioning increased the cell survival rate and decreased the lactate dehydrogenase leakage under acute anoxia, in addition to maintaining MMP at a relatively higher level and inducing bcl 2 overexpression during anoxia. The results suggest that hypoxic preconditioning can enhance the tolerance of hypothalamic cells to anoxia, and the underlying mechanism may be related to increased stability of MMP. Overexpression of bcl 2 induced by hypoxic preconditioning may play a role in sustaining the stability of MMP. [WT5HZ]