摘要
采用蒙古沙土鼠双侧颈总动脉阻断前脑缺血模型 ,以放射自显影 (反向磷酸化 ,back phosphorylation)及免疫印迹 (Westernblotting)法体外测定缺血时纹状体DARPP 32磷酸化水平和蛋白含量的变化。结果表明 ,短暂性缺血纹状体DARPP 32的免疫学活性和蛋白含量无明显改变。在缺血 10min内 ,随缺血时间的延长 ,体外DARPP 32的 [32 P]的掺入量在缺血 5min时升高 ,在缺血 2、7、10min时均降低 ,而反向磷酸化的测定结果表明体内DARPP 32磷酸化水平增高 ,说明缺血可诱导DARPP 32磷酸化水平变化。
Using the mongolian gerbil model of transient forebrain ischemia by bilateral carotid arteries occlusion, the levels of phosphorylation and the amount of DARPP 32 in striatum were measured by back phosphorylation and Western blotting assay in vitro . Transient forebrain ischemia had no effect on the immunoreaction of DARPP 32 in striatum, but the state of phosphorylation of DARPP 32 showed a significant change. The [ 32 P] phosphate incorporation in DARPP 32 decreased after 2, 7 or 10 min of ischemia, but increased after a 5 min ischemia. In contrast, the result of back phosphorylation in vivo was opposite to that obtained in vitro .[WT5HZ]
出处
《生理学报》
CAS
CSCD
北大核心
2001年第2期108-110,共3页
Acta Physiologica Sinica
基金
ThisworkwassupportedbytheProvincialNaturalScienceFoundationofJiangsu
(BK9715 3)andtheNaturalScienceFoundationofEducationCommit
关键词
脑缺血
蒙古沙土鼠
DARPP-32
反向磷酸化
纹状体
cerebral ischemia
mongolian gerbil
DARPP-32
back phosphorylation
striatum
Western blotting
