BALB/c小鼠胚胎干细胞系的建立及其嵌合体小鼠的获得

ESTABLISHMENT OF ES CELL LINE DERIVED FROM BALB/c MICE AND SUCCESS OF MAKING UP CHIMAERIC MICE

目的:建立BALB/c小鼠胚胎干细胞系,并用于制作嵌合体小鼠。方法:从BALB/c小鼠囊胚内分离培养内细胞团块。建系后,进行C_(57)BL/6小鼠受体囊胚腔注射,制作嵌合体小鼠。结果:建立了我国第一株BALB/c小鼠胚胎干细胞系。该细胞系具有典型的ES细胞形态,碱性磷酸酶强阳性,核型正常以及具有分化为三种胚层组织的能力,并已产生5只嵌合体小鼠。结论:建立的BALB/c小鼠胚胎干细胞系具有胚胎干细胞的各种特点,可用于体内外诱导分化研究。在进一步观察生殖系嵌合情况后,决定是否可应用于基因打靶等转基因动物的制作。

Purpose: To establish an embryonic stem(ES)cell line derived from BALB/c mice and make chimaeric mice. Method: Isolation and culture inner cell mass( ICM.)from BALB/c mice blastocysts. After stable proliferation and propagation, we create chimaeric mice by injecting ES cells into blastocysts of C57BL/6J mice. Results: We have established the first embryonic stem cell line derived from BALB/c mouse in China. It has stably maintained in an undifferentiated state with a typical morphology of ES cell clone, expressing the embryonic stem cell marker alkaline phosphatase, and displaying a normal diploid karyotype. Five chimaeric mice have been obtained. Conclussion: The established embryonic stem cell line express all characteristics of the ES cells. It can be used on differential research in vivo and in vitro. After further observation of its germline transmission, we 11 deside whether it can be applied to make-up transgenic and knock out animal.