扩增未知序列DNA片段的PCR技术研究进展

The advance in the research on amplifying Unknown DNA sequence

198 5年 ,Mullis等人发明了PCR技术 ,短短十余年间 ,这一技术得到迅速发展和应用 ,已由扩增已知基因发展到扩增未知基因。本文旨在介绍利用PCR技术扩增未知序列DNA片段的最新进展及这些技术在基因克隆研究中的应用。

The PCR technology has been advanced rapidly since it was invented by Mullis et al in 1985,and it has been widely applied in molecular biology study.The article reviewed the recent progresses of technology in amplifying unknown DNA sequence and in gene cloing.\;