重要禾谷类植物转基因研究

Transgenic Researches of Important Cereal Species

与双子叶植物相比 ,禾谷类植物的离体培养和再生主要采用一些胚性组织 ,对它们的转基因研究发展相对比较缓慢。 90年代以来 ,离体培养方面的经验被有效地融入DNA转移技术 ;通过筛选和再生少量的转化细胞 ,已得到了不少可育的小麦、水稻、玉米、大麦等禾谷类转基因植物。本文着重综述了禾谷类植物转化方法和选择体系方面的研究现状 ,同时讨论了该研究方向的未来发展趋势。

Compared with dicotyledonous plants,the development of transgenic researches in cereal plants is relatively slow.The reason is that the source materials in in vitro culture and regeneration of them are mainly restricted to some embryogenic tissues.From 1990s,the experience in in vitro culture has been fused successfully into the techniques of DNA transfer.Through selection and regeneration of a few cells integration the foreign genes,a series of fertile transgenic cereal plants,including wheat,rice,barley,maize,etc.have been obtained.The present paper mainly summarizes the current situation of transformation method and selection system in cereals,and discusses the future trend of this research direction.\;