摘要
通过对敲除磷脂酶 C-γ1 基因的鼠成纤维细胞株及其转染磷脂酶 C-γ2 基因后的迁移指标的统计学分析 ,揭示出磷脂酶 C-γ1 和γ2 在 PDGF引起的细胞迁移信号传导中均有正调控作用 ,前者作用显著而后者作用微小 ,并且仅在磷脂酶 C-γ1 功能缺失的条件下方可发挥出来 ;同时进一步证明敲除磷脂酶 C-γ1 基因的细胞株中 ,未见出现磷脂酶 C-γ2 的代偿 ,说明磷脂酶 C-γ2 与 γ1 在功能上不可相互取代 ,反映了一种信号可由多种途径传导即细胞信号传导的交互性。
By statistical analysis of the migration indices of phospholipase C γ 1 gene knocked out and phospholipase C γ 2 gene vector transfected fibroblasts, we found that phospholipase C γ 1 and phospholipase C γ 2 both shew a PDGF induced positive regulator effect on the migration signal of fibroblast. The effect of the former was significantly stronger than the later and phospholipase C γ 2 played an active role only under the condition with functional deficiency of phospholipase C γ 1. This study also showed no phospholipase C γ 2 compensation in phospholipase C γ 1 gene knocked out cell line, which suggested that these enzymes could bring with independent functions which implied complexity and redundancy in such multiple signal transduction pathway.
出处
《生命科学研究》
CAS
CSCD
2001年第1期33-38,共6页
Life Science Research
基金
国家自然科学基金项目!(39870 381)
广东省自然科学基金资助项目!(980 2 13)
