长白山区莱姆病调查与实验室分析

Survey and Laboratory Analysis on Lyme Disease in Changbaishan

目的 :了解长白山区莱姆病传播媒介 -全沟硬蜱的地理位置、分布及其病原携带情况。方法 :用酶联免疫吸附试验、间接免疫荧光试验检测莱姆病特异IgG抗体。 结果 :长白山区抓捕的 6 2 0只硬蜱 ,全沟硬蜱为5 93只 ,占总数的 95 .80 % ,用BSK培养基 33℃培养 ,分纯后共获得纯阳性伯氏疏螺旋体培养物 2 43株 ,用兔抗美国B31特异性多克隆抗体和针对莱姆病螺旋体种属特异单克隆抗体 ,间接免疫荧光抗体试验进行鉴定 ,证明其单克隆抗体反应与美国菌株稍有差异 ,与国内分离株M7相同。血检 82 6人 ,IgG、IgM≥ 1∶12 8(抗体滴度 )有 49例 ,阳性率为 5 .93% ;IgG、IgM≥ 1∶6 4(抗体滴度 )有 82例 ;二项合计为15 .85 %。结论 :长白山区是莱姆病重要疫源地 ,人群中有莱姆病感染存在 。

Objective:To investigate the distribution of Ixodes persulcatus and the relation with Lyme disease.Methods:To detect the specific IgG by means of ELISA and indirect immunofluorescence.Results:We investigated on geographical conditions, distribution of Ixodes persulcatus and isolated Lyme disease spirochetes in Changbaishan. We conducted the survey of specific IgG by means of ELISA and indirect immunofluorescence and found that there were 593(95.80%) Ixodes persulcatus in 620 Ixodes ang got 243 positive Borrelia burgdorferi culture. By means of hemospasia survey the sera of 826 mountain residents were tested for antibodies, 131(15.85%) mountain residents had significant IgG titers, including 49(5.93%) IgG, IgM≥1∶128 and 82(9.82%) IgG,IgM≥1∶64. Conclusion:It was comfirmed that Changbaishan is an important epidemic focus. There are infections of Lyme disease among the mountain residents in Changbaishan.