蚊体内马来丝虫幼虫的基因检测

Studies on Gene Detection of Brugia malayi Larvae in Mosquito

目的 :探索蚊体内马来丝虫幼虫基因检测的新方法 ,并用于丝虫病监测。方法 :利用基因工程技术合成马来丝虫寡核苷酸片段 ,经32 P标记后作为探针 ,以斑点杂交法检测蚊体内马来丝虫幼虫。结果 :该探针可从多种标本中特异地检出马来幼丝虫DNA ,敏感性为 2ng靶DNA量 ,蚊体内含 1条幼虫即可出现阳性杂交。结论 :该技术具有良好的实用性 。

Objective:To establish a new method for gene detection of Brugia malayi larvae in mosquito and use it in filariasis surveillance. Methods:Oligonucleotide fragment of Brugia malayi synthesized by genetic engineering technique was labeled with 32 P and used as probe. The B.malayi larvae in mosquito was detected with dot blot. Results: Brugia malayi larvae DNA could be specifically detected in different samples with this probe and the sensitivity was 2 ng target DNA of B.malayi . Even only one larvae in mosquito could be detected after hybridization. Conclusions:This method is practicable and reliable for gene detection of Brugia malayi larvae in mosquito.