埋植17α-甲基睾酮诱导点带石斑鱼性转化技术

Technique of implanting 17α-methyltestosterone to induce the sex reversal of Epinephelus malabaricus.

论述了埋植 17α 甲基睾酮诱导点带石斑鱼 (Epinephelusmalabaricus)性转化的方法 ,包括药物的处理、用量、手术操作等 .3龄的雌性点带石斑鱼经过 2次埋植外源激素手术转化为功能性雄鱼 ,139d后 5 8%可挤出精液 ,30 1d后 10 0 %可挤出精液 .功能性雄鱼用于人工繁殖 ,亲鱼自然产卵 ,1999～ 2 0 0 0年 2年内共孵出仔鱼710 3万尾 .所得仔鱼用于鱼苗培育 ,得到发育正常的幼鱼 .认为埋植外源激素诱导点带石斑鱼完成性转化的方法简便可行 ,能满足规模化人工繁殖的需要 .

This paper discussed the method of implanting 17α-methyltestosterone to induce the sex reversal of Epinephelus malabaricus, which included chemical treatment, dosage and operating on the fish. After twice implanting of exogenous hormone, the mature females of 3 years old were transformed into functional males. Sperm could be squeezed from 58% of the fish on 139 days after operating,and from 100% on 301days. When the functional males were used for artificial breeding, the parent fish spawned in a natural way, and 71.03 million larvae were hatched in the two years of 1999～2000. The larvae were used for rearing of fish fry, from which normally growing young fish was get.It is believed that the method of implanting exogenous hormone to induce the sex reversal of E.malabaricus is easy and stable, which can satisfy the requirement of artificial breeding in factories.