血虚模型动物制备及当归补血汤的作用研究

Preparation of Blood-Deficient Model and Effects of Tangkuei Blood-Supplementing Decoction on Hemopoiesis

目的 :对原有的血虚模型进行改进 ,并观察当归补血汤的作用。方法 :采用乙酰苯肼 6 0mg/kg和环磷酰胺16 0mg/kg联合造成小鼠的血虚状态。取血测定小鼠的外周血象 ,取股骨测定骨髓有核细胞数量 ,及以电镜观察骨髓超微结构 ,观察模型小鼠的游泳时间、体温及放免法测定血浆cAMP、cGMP的比值。同时观察了当归补血汤改善血虚证的作用。结果 :当归补血汤能显著增加模型小鼠的红细胞、白细胞、骨髓有核细胞的数量 ,改善网织红细胞在外周血中的比例及骨髓超微结构 ,并能延长模型小鼠的游泳时间、升高体温、提高血浆cAMP/cGMP比值。结论 :实验制备的血虚动物模型属于血虚证的范围 ,反映了血虚证的主要特征 ,可作为血虚证模型之一。

Objective: To prepare a blood deficient model and study the effect of Tangkuei Blood Supplementing Decoction on hemopoiesis in this model. Methods: This model was made in mice by i.p. an accumulate doses of 60mg/kg acetylphenylhydrazine (APH) and 160mg/kg cyclophosphamidum (CY). The RBCs, WBCs, reticulocytes and bone marrow nucleated cells (BMNC) were counted, the micro structure of bone marrow was observed. The swimming time, the body temperature and the plasma cAMP, cGMP levels were measured. The effects of Tangkuei Blood Supplementing Decoction by p.o. administration on promoting the hemopioetic function were observed with the model mice. Results: Tangkuei Blood Supplementing Decoction could remarkably increase RBC, WBC, BMNC, improve the proportion of reticulolytes in peripheral blood and the micro struture of bone marrow, prolony the swimming time, raise the body temperature and the specific value of cAMP/cGMP. Conclusion: The model exhibits the main features of blood deficiency, and can be used as one of models of blood deficiency. Tangkuei Blood Supplementing Decoction can obviously improve the dual deficiention of qi and blood of model mice by supplementing qi and blood.