神佗伤湿灵中樟脑与薄荷脑的气相色谱测定

Determination of Camphor and menthol in Shentuo Shangshiling by Gas Chromatography

目的 :用气相色谱法测定神佗伤湿灵中樟脑及薄荷脑的含量。方法 :以10 %PEG20M为固定相 ,ShimaliteW(NAW)60～80mesh为担体 ,萘为内标。结果 :樟脑及薄荷脑浓度分别在1 41mg/ml～11 30mg/ml和1 40mg/ml～11 21mg/ml范围内 ,与A对/A内 之间呈线性关系。回归方程分别为 :樟脑Y=0 19969C -0 00244(r=1 0000) ;薄荷脑Y=0 22175C-0 00260(r=0 9999)。加样回收率分别为 :樟脑100.56%(n=6 RSD=0 45%);薄荷脑101 01%(n=6,RSD=0 79%)。结论 :气相色谱测定法测定樟脑与薄荷脑快速、简便、重现性好 ,结果准确、稳定、可靠。

Purpose: To determine the contents of camphor and menthol in Shnetuo Shangshiling by gas chromatography. Methods: 10% PEG-20M was used as stationary phase, Shimalite W(NAM) 60～80 mesh as supporter and naphthalene as internal standard. Results: The concentrations of camphor and menthol were linear to ratios of their peak areas to the naphthalene's area in the range of 1.41mg/ml/～11.30mg/ml and 1.40mg/ml～11.21mg/ml respectively. The regressive equations were:camphor Y=0.19969 C-0.00244(r=1.0000,n=5),menthol Y=0.22175 C-0.00260(r=0.9999,n=5). The mean added-recovery rates were: camphor 100.56%(n=6, RSD=0.45%); menthol: 101.01%(n=6, RSD=0.79%). Conclusions: Being simple, fast and duplicable in operation, gas chromatography is a good method to determine the content of camphor and menthol with accurate, stable and reliable results.