兔视网膜静脉阻塞眼视网膜新生血管组织中血管内皮生长因子mRNA的表达

VEGF mRNA expression in retinal neovascular tissue in rabbit eyes with retinal vein occlusion

目的 探讨血管内皮生长因子 (vascular endothelial growth factor,VEGF )在视网膜新生血管组织的发生发展中的参与机制。 方法 采用氩激光直接光凝法封闭兔眼视网膜静脉建立视网膜静脉阻塞 (retinal vein occlusion,RVO)模型。利用组织原位杂交法观察缺血视网膜组织及新生血管组织中 VEGFm RNA的表达。 结果 缺血视网膜及新生血管组织中有不同程度的 VEGF m RNA表达 ,表达的程度以视网膜新生血管组织中最强。VEGF m RNA的表达部位与视网膜组织缺血的分布具有一定的对应性。 结论 VEGF在视网膜血管增生性病变的发生中可能具有重要的参与机制。

Objective To evaluate the possible role of vascular endothelial growth factor (VEGF) in the pathogenesis of retinal neovascularization resulting from retinal ischemia. Methods Retinal vein occlusion of rabbits model was established with argon laser photocoagulation to retinal vein.The VEGF mRNAexpression was observed in ischemic retina and retinal neovascular tissue with in situ hybridization technique. Results VEGF mRNA expression was detected in inner ischemic retina and retinal neovascular tissue that were corespondent to the distribution of retinal ischemia. The strongest VEGF mRNA expression was detected in retinal neovascular tissue. Conclusion VEGF might play an important role in the pathogenesis of vascular proliferative retinopathy.