单疱病毒性角膜炎患者外周血几种细胞因子水平的改变及临床意义

Changes of cytokines in peripheral blood of patients with herpes simplex keratitis and the clinical significance

探讨几种细胞因子在单疱病毒性角膜炎 ( herpes simplex keratitis,HSK)发病机理中的作用及意义。方法 :用双抗体夹心法酶联免疫吸附试验 ( EL ISA)对 5 1例 HSK患者 (其中活动期 33例 ,非活动期 18例 )作了外周血白细胞介素 6 ( IL - 6 )、白细胞介素 8( IL - 8)、α肿瘤坏死因子 ( TNF-α)、α干扰素 ( TFN-α)水平观察 ,并用同样方法以 5 0名正常献血员作正常对照。结果 :HSK活动期患者的 IL- 6、IL- 8、TNF- α测定值与 HSK非活动期患者和正常对照组相比明显增高 ;IFN- α与 HSK非活动期患者和正常对照组比较显著降低 ,差异均有非常显著性 ( P<0 .0 1) ;基质型 HSK患者与上皮型 HSK患者 IL- 6、IL-8、TNF- α相比 ,差异有非常显著性 ( P<0 .0 1)。结论 :HSK患者正常免疫功能受抑制及几种细胞因子水平异常可能导致 HSK的发生与发展 ,IL - 6、 IL - 8、 TNF-α、 IFN-α的测定 ,可作为

to study the roles of cytokines in the pathogenesis of herpes simplex keratitis(HSK)and their significance.Methods:The cytokines(IL-6,IL-8,TNF-α,IFN-α)in 51 patient with HSK(including 33 cases of active keratitis and 18 inactive keratitis)were measured by sandwich ELISA technique.50 normal donors were used as control.Results:In the comparison with the cases of inactive keratitis and the control group,there were significantly increased IL-6,IL-8,and TNF-α levels in active keratitis(P<0 01),and markedly reduced IFN-α in inactive keratitis(P<0 01);therer were difference IL-6,IL-8,TNF-α between the epithelial case and the basement(P<0 01).Conclusions:The pressing immunity and unusual cytokine level of the patients probably lead to both causing and development of HSK.The determination of IL-6,IL-8,TNF-α and IFN-α may provide referential value for studying of treatment of HSK and evaluating its prognosis.