原位杂交检测HOXA-10基因在人子宫内膜的表达

The expression of HOXA-10 in the endometrium of fertile women by in situ hybridization

目的 :探讨正常生育妇女月经周期不同时期子宫内膜腺上皮细胞、间质细胞中HOXA-1 0基因的表达。方法 :采用原位杂交的方法。结果 :HOXA-1 0基因在子宫内膜腺上皮细胞、间质细胞均有表达 ;中晚分泌期的表达高于增殖期及分泌早期 ,且间质细胞的表达高于腺上皮细胞。结论 :( 1 ) HOXA-1 0基因在分泌中期子宫内膜高表达提示其可能在人胚胎着床过程中起某种作用。 ( 2 ) HOXA-1 0基因可能在人子宫内膜蜕膜化过程中有重要作用。

Objective: To study the expression of HOXA-10 mRNA in the glandular epithelial cells and stromal cells of endometrium of fertile women during the menstrual cycle.Method: In situ hybridization method.Results: (1)HOXA-10 mRNA levels can be detected in the glandular epithelial cells and stromal cells of endometrium in fertile women during the menstrual cycle. (2)HOXA-10 mRNA levels were significantly higher in the mid-and late-secretory phase than those in the proliferative phase and early secretory phase (P<0.01). HOXA-10 mRNA levels in endometrial stromal cells at midsecretory and late secretory were significantly higher than those in glandular epithelial cells (P<0.01) Conclusion: (1)High expression of HOXA-10 around the time of implantation indicates that HOXA-10 may involve in implantation. (2)HOXA-10 has an important function in the decidulization of endometrium.