一氧化氮对全胃肠外营养肝脂肪变性的保护作用

Protection of nitric oxide for hepatic steatosis caused by total parenteral nutrition in rats

目的 探讨一氧化氮 (NitricOxide ,NO)对全胃肠外营养 (TotalParenteralNutrition ,TPN)引起的肝脂肪变性的作用。方法 30只Wistar大鼠随机分为 5组 :A组 ,正常对照 ;B组 ,TPN ;C组 ,TPN +精氨酸 ;D组 ,TPN +NG 硝基 L 精氨酸甲酯 (NG nitrio L argininemethylester,L NAME) ;E组 ,TPN +精氨酸 +L NAME。实验 7d后测肝功能 ,肝内脂肪 ,肝脏NO含量及NOS活性和肝脏组织学检查。结果 B组肝内脂肪甘油三脂 (μmol/g)及胆固醇 (μmol/g)分别为 (39 3± 2 4、13 1± 1 1)较A组(6 9± 0 8、5 6± 0 6 )显著增加 (P <0 0 5 ) ,D组肝内脂肪 (4 9 7± 6 2、14 1± 1 7)增加较B组更显著(P <0 0 5 ) ,C组肝内脂肪 (18 2± 4 4、7 3± 3 1)较B组明显减少 (P <0 0 5 ) ,E组肝内脂肪 (35 8±8 8、10 0± 2 4)与B组相似 (P >0 0 5 ) ,肝内NOS活性及分布与肝内脂肪含量及分布相平行。

Objectives To investigate the effect of nitric oxide(NO) on hepatic steatosis caused by total parenteral nutrition(TPN) in ratsMethods Thirty Wistar rats were randomly divided into five groups: Group A, normal control; Group B, TPN group;Group C, TPN plus L arginine; Group D, TPN plus N G nitrio L arginine methyl ester(L NAME,NOS inhibitor); Group E, TPN plus L arginine and L NAME. At the seventh day, liver function tests were performed and the livers were resected to test the lipid and nitric oxide content, and nitric oxide synthase activity.[WT5”HZ] Results [WT5”BZ] The hepatic lipid content 〔triglycerid(μmol/g), cholesterol(μmol/g)〕in group B significantly increased compared with group A (39 3±2 4、13 1±1 1 vs. 6 9±0 8、5 6±0 6)( P <0 05). That was higher in group D(49 7±6 2、14 1±1 7)than in group B( P <0 05), whereas that was lower in group C(18 2±4 4、7 3±3 1)than in group B( P <0 05).The activity and distribution of NOS in the liver were associated with the content and distribution of hepatic lipid Conclusions NO produced by the liver may protect hepatic steatosis caused by total parenteral nutrition in rats