短肠大鼠结肠对^(15)N-甘氨酸的吸收研究

^(15)N-glycine absorption in the colon of the short bowel rats

目的 探讨短肠大鼠的结肠对氨基酸代偿吸收能力。方法 切除 80 %小肠的短肠大鼠 ,经肠喂养 2 1天后 ,测定结肠对15N 甘氨酸的吸收率和血15N 甘氨酸丰度及形态学改变。结果正常大鼠 3h水吸收为 (1± 0 5 )ml,对15N 甘氨酸无吸收 ,血中15N 甘氨酸丰度为零。短肠大鼠 3h水吸收为 (2 6± 0 82 )ml(P <0 0 5 ) ,15N 甘氨酸为 (4 39± 1 2 ) % (P <0 0 1) ,血中15N 甘氨酸丰度为(0 6 14± 0 5 17) % (P <0 0 1)。形态学检测显示 :喂养组与对照组相比结肠直径差异有显著意义(P <0 0 5 ) ,而结肠壁厚度、粘膜厚度、皱襞高度和皱襞表面积差异有极显著的意义 (P <0 0 1)。结论 短肠大鼠结肠对水和15N 甘氨酸有一定代偿性吸收 。

Objective To investigate 15 N glycine absorption in the colon of the short bowel rats. Methods Rats undergoing resection of 80% small intestine and were treated by enteral nutrition for 21 days. The concentration of 15 N glycine in perfusate samples was measured by the technique of HPLC. The atom percent enrichment of 15 N glycine in blood plasma was measured by the technique of isotopic dilution method Results fter 3 hours, the colonic water absorption was 1±0 5 ml, 15 N glycine absorption was zero, and the atom percent enrichment was near zero in normal group, vs. (2 6±0 82) ml ( P <0 05), (4 39±1 2)% ( P <0 01), and (0 614±0 517)% ( P <0 01) in short bowel rats, respectively. The mucosal thickness, villous height and surface area of the colon in enteral nutrition group were greater than that in controls ( P <0 01).[WT5”HZ] Conclusion [WT5”BZ] The short bowel rats had significant colonic amino acids and water absorption adaptation, though not enough to fulfil the need of the body.