放射自显影术对机体不同组织的蛋白质代谢研究

A comparative study on protein metabolism in various tissues by autoradiography

用放射自显影术在预先注入体内蛋白质标记前身物^(35)S-蛋氨酸后,研究在不同脏器和细胞水平上蛋白质的生物合成部位和合成的相对程度,从定位、定量的角度分析在体内各脏器组织中蛋白质生物合成能力的差异。研究结果发现,以肝组织中的蛋白质生物合成能力最高,其次为肾脏、脾脏、大脑以及骨髓的幼稚细胞。而一些肚器组织如心脏、肺脏和骨骼肌等虽具有重要的生理活性,但其体内的蛋白质的生物合成却并不旺盛。

By the use of autoradiographic technique, the incorporation of 35S-methionine in tissue protein has been utilized as an index of tissue protein synthesis. It was found that 35S-methionine incorporates rapidly in immature cells of bone marrow, liver, kidney and spleen. In spite of their important physiological functions, heart, lung and skeletal muscle have their proteins synthesized at low speed.