同种异体软骨细胞-聚羟基乙酸复合物修复喉软骨缺损

Laryngeal cartilage defect repair with allogenic chondrocyte－ PGA complex

目的探讨同种异体软骨细胞－聚羟基乙酸 (PGA)复合物在有免疫力的动物体内对喉甲状软骨的修复能力。方法消化分离乳兔肋软骨，获取种子细胞，体外培养，收养第 2～ 3代培养细胞，接种于经多聚赖氨酸处理后的 PGA三维可降解生物支架料上。结果复合物体外培养 7～ 10d，可见呈蜘蛛网状基质产生。术后 4周，修复区愈合良好，无瘢痕及坏死现象；组织学观察可见愈合面有软骨细胞生成和基质分泌。结论软骨细胞－ PGA复合物可用于喉软骨缺损的修复，修复区愈合良好，无明显免疫排斥反应。

Objective To explore the ability to repair laryngeal cartilage defects using chondrocyte－ polyglycolic acid(PGA)complexes.Method Chondrocytes were isolated from costa－ cartilage of infant rabbits byⅡ type collagenase digestion.they were cultured in vitro for 2～ 3 generations with Ham－ F12,and then the cells were cultured in PGA scaffolds.Result cartilaginors matrix was observed 7～ 10 days after culturing.4weeks after operation,laryngeal cartilage defects were initally filled with new cartilage.No scar and necrosis in operative areas were found.More chondrocytes and matrix were observed in reparative areas.Conclusion Chondrocyte－ PGA complex can be used as reparative material for laryngeal thyroid cartilage defect.