OK-432联合IL-2对C_(57)BL/6小鼠Lewis肺癌的抑制作用及c-fos蛋白的表达

Inhibition Effects of OK-432 Combined with IL-2 on Lewis Lung Cancer Growth and c-fos Protein Expression in C_(57) BL/6 mice

目的研究 OK- 432与 IL- 2抑制近交系小鼠 C5 7BL/ 6 L ewis肺癌 (L L C)的生长及 c- fos蛋白的表达。方法以 C5 7BL/ 6荷瘤 L L C小鼠为模型 ,观察 OK- 432联合 IL- 2对肿瘤发生、生长、转移的影响 ,体内对肿瘤细胞的作用及用抗鼠 c- fos单克隆抗体进行 SABC免疫组化技术半定量测定 c- fos在 L ewis肺癌原发灶中的表达。结果二者联用对肿瘤体积和重量的抑制作用明显增强 (P<0 .0 1) ,增强抑瘤率 (P<0 .0 5 ) ,肺转移灶数目明显减少。电镜下显示肿瘤灶中出现许多凋亡细胞 ,瘤细胞核染色质浓集成块 ,在核膜内呈新月形或环形排列 ,核膜清楚 ,瘤细胞内质网扩张 ,线粒体肿胀。c- fos在联合用药组与对照组比较明显减少 (P<0 .0 1)。结论 OK- 432与 IL- 2有明显的协同抗肿瘤活性 ,其杀瘤过程主要为对瘤细胞的直接或间接攻击 ,和促进体内对多种反应细胞的相互作用 ;部分原因可能阻抑 c- fos癌基因的表达 ;提示 OK- 432联合 IL-

Objective To study inhibition effects of OK 432 and IL 2 on inbred C 57 BL/6 mice bearing Lewis lung cancer(LLC) growth and c fos protein expression.Methods Through establishing C 57 BL/6 mice bearing LLC model,we observed effects of OK 432 and IL 2 on tumor occurrence,growth,metastasis,tumor cell changes in vivo ,and the expression of c fos in primary tumor tissues with SABC immunohistochemical semiquantitative method.Results Combination administration significantly enhanced inhibition effects on tumor volume and weight versus control( P <0 01),and reduced the number of pulmonary metastasis loci.A lot of apoptosis cell emerged in the tumor loci under electronmicroscope.The expression of c fos significantly decreased in combination group versus that in control group( P <0 01).Conclusion OK 432 and IL 2 possessed obviously synergetic anti tumor effect,which mainly through attacking the tumor cell directly or indirectly,improved some kinds of cell interaction and partly by inhibiting c fos ongene expression;In a word,OK 432 and IL 2 might be an efficient biotherapy means for cancer patients in clinical.\;