不平衡氨基酸对肿瘤生长的影响

Influence of imbalance amino acid solutions on tumor growth in tumor-bearing rats

目的 :比较几种不平衡氨基酸营养支持对肿瘤生长的影响。 方法 :荷Walker 2 5 6癌肉瘤SD大鼠 ,空肠喂饲营养制剂 10天 ,根据所喂饲制剂中氨基酸组成的不同 ,分为A组 (平衡氨基酸组 )、B组 (增量精氨酸不平衡氨基酸组 )、C组 (去甲硫氨酸不平衡氨基酸组 )、D组 (去缬氨酸不平衡氨基酸组 )、E组 (增量精氨酸、去甲硫氨酸和缬氨酸不平衡氨基酸组 )。以肿瘤体积增长速度 ,肿瘤重量 ,肿瘤重量 /尸重 ,肿瘤组织PCNA指数 ,肿瘤细胞周期各时相百分比 ,以及DNA倍性作为观察肿瘤生长速度的指标。 结果 :上述各指标值显示 :B、C、D组与A组相比 ,除DNA倍性外 ,其余各指标值均非常显著降低 ,但B、C、D组间比较无显著差异。E组与B、C、D组相比较 ,所有指标值均显著降低。 结论 :平衡氨基酸促进肿瘤生长 ;增量精氨酸、去甲硫氨酸和去缬氨酸三种不平衡氨基酸对肿瘤生长有同等抑制作用 ;增量精氨酸、去甲硫氨酸和去缬氨酸复合不平衡氨基酸对肿瘤生长抑制作用更显著。

Objectives:To study effects of nutrition support of imbalance amino acids solutions on tumor growth in tumor bearing rats. Methods:Different nutritional solutions had been used for 10 days by jejunostomy in rats bearing Walker 256 carcinosarcoma.The tumor bearing rats were divided into five different groups according to formulas of amino acid composition in nutritional solutions respectively:group A(balance amino acid solution),group B(arginine enriched amino acid solution),group C(methionine depleted amino acid solution),group D(valine depletedamino acid solution,and group E (complex amino acids solution which was arginine enriched,methionine depleted and valine depleted).Indices of tumor growth were investigated. Results:The levels of above indice except DNA ploidy were significantly lower ingroups,B、C and D than those in group A,but there were no significant differences among group B、C and D.All tumor growth indices of group E were significantly less than those of group B、C and D. Conclusions:Balance amino acid solution can stimulate tumor growth.Arginine enriched amino acid solution、methionine depleted amino acid solution and valine depleted amino acid solution,can inhibit tumor growth seperately;Complex amino acid solution which is arginine enriched,methionine depleted and valine depleted has stronger inhibitory effect on tumor growth.