抗肿瘤坏死因子α单克隆抗体对急性胰腺炎大鼠肠道完整性的影响

Influence of anti-TNFαMcAb on intestinal integrity in rats with acute pancreatitis

目的 :探讨抗肿瘤坏死因子α单克隆抗体 (anti-TNFαMcAb)对急性胰腺炎大鼠所致肠道完整性的影响。 方法 :SD大鼠 32只 ,制备急性胰腺炎模型后 ,随机分为对照组 (n=16 )及实验组 (n =16 ) ,对照组给予全肠外营养支持 ,实验组给予相同配方的全肠外营养支持 ,并予anti-TNFαMcAb静脉内注射。于建立急性胰腺炎模型后第 1天及第 5天处死 ,检测大鼠肠道通透性、吸收功能、空肠粘膜湿重、微绒毛高度和面积、双糖酶活性以及肠道细菌移位率。 结果 :两组动物间肠道通透性、吸收功能、空肠粘膜湿重、微绒毛高度和面积、双糖酶活性以及肠道细菌移位率均无显著差别。 结论 :Anti-TNFαMcAb对急性胰腺炎大鼠肠道完整性无明显影响。

Objectives:To evaluate the influence of anti TNFα monocolonal antibody (anti TNFαMcAb) on intestinal integrity in rats with acute pancreatitis under total parenteral nutrition. Methods:Acute pancreatitis model was induced in 32 male Sprague-Dawley rats. They were randomly divided into control group (n=16) and experiment group (n=16). Animals in control group received total parenteral nutrition (TPN). Animals in experiment group received both TPN were fed the identical TPN formula received by control group and injections of anti TNFαMcAb. Rats were sacrificed on day 1 and day 5 after the induction of acute pancreatitis for measurements of intestinal permeability, absorptive capacity, mucosal wet weight, villus height and area, activities of sucrase and maltase in jejunum and bacterial translocation. Results:There was no significant difference between the two groups in intestinal permeability, absorptive capacity, mucosal wet weight, villus height and area, activities of sucrase and maltase in jejunum and bacterial translocation. Conclusions:Anti TNFαMcAb has not significant influence on intestinal integrity in rats with acute pancreatitis under TPN.