羊房室结覆盖层的组织学观察和定量分析

Histological observation and quantitative analysis of overlay layer of atrioventricular node in heart of goats'

目的 了解羊房室结覆盖层的形态学特点及细胞成分 ,为探讨房室传导机理和心血管实验提供形态学参考。方法 石蜡切片 ,HE染色 ,光镜观察 2 3只正常杂种山羊房室结的覆盖层 ,并用HPIAS -10 0 0高清晰度彩色图文分析系统定量分析。结果 羊房室结有典型的覆盖层 ,青年羊组覆盖层平均厚度为 0 .2 8± 0 .13mm ;老年羊组平均厚度为 0 .5 1± 0 .2 3mm ,差异有显著性意义 (P <0 .0 1)。图象分析结果显示覆盖层细胞与一般心肌细胞的多项参数有显著差异。结论 羊房室结有典型的覆盖层 ,其厚度随年龄增长而增加 ,其细胞属移行细胞。

Objective To study the morphological features and the cell composition of atrioventricular(AV) node overlay layer, in order to provide morphological reference for cardiavascular experiment and for the mechenism of atrioventricular conduction. Methods Using technique of paraffin section and H.E staining, 23 AV node and its overlay layer of goats heart were observed under the microscope. High-clear color image analysis system (HPIAS-1000) was used to do the quantitative analysis. Results The AV node of goats was endowed with typical overlay layer, its thickness was 0.28±0.13 mm in the young group and 0.51±0.23 mm in the old group. There was statistics difference between the two groups. The quantitative analysis indicated: there was difference of some parameters between the overlay cells and cardiac muscle cells. Conclusion The atrioventricular node of goat has typical cover layer. The thickness of overlay layer increase with the raising of age, and the cells are referred to transitiolnal cell.