小鼠四氯化碳急性中毒性肝损害模型制备方法探讨

Research on the preparation method of acute liver damage mice model caused by carbon tetracholoride poisoning

目的 探讨灌胃、皮下注射、腹腔注射 3种不同染毒途径对小鼠四氯化碳急性中毒性肝损害程度的不同。方法 昆明种小鼠 6 0只 ,随机分为 6组 (n =10 ) ,灌胃、皮下注射、腹腔注射 3种给药途径分别设染毒组、对照组 ,共 6组 ,给药 2 4h后 ,每组每只小鼠由腹腔注射 0 .4%硫喷妥钠 0 .1ml·10 g-1,观察小鼠麻醉维持时间 ,然后处死小鼠 ,肉眼观察肝脏并作病理检查。结果 灌胃和皮下注射染毒均能引起小鼠四氯化碳急性中毒性肝损害 ,与各自对照组比较差异显著 (P <0 .0 1)。结论 可以根据所研究药物的不同 ,选择灌胃、皮下注射染毒来制造小鼠肝损害动物模型。

Objective To research the difference in degrees of acute liver damage in mice models caused by carbon tetracholoride (CCl 4) with 3 routes of administration, e.g.ig, sc and ip. Methods Sixty of the Kunming mice were casually divided into six groups (n=10), including three liver damage groups and three normal groups. The carbon tetracholoride were given through ig, sc, ip. At 24h after injection, each mice of all the six groups was injected 0.1 ml·10g -1 of 0.4% thiopental sodium through ip. The maintain time of anaesthesia was observed, then the mice was killed. The liver of animal pattern with naked eyes and pathological histology test was observed. Results Both ig and sc could cause acute liver damage of mice by CCl 4, and the degree of liver damage in both groups was higher than that of the normal group (P<0.01). Conclusion The preparation method of acute liver damage mice models caused by CCl 4 can be made by choosing ig and sc according to the different medicines studied.