摘要
目的 :研究脊髓损伤后脊髓神经元的酶学变化 ,并探索睫状神经营养因子 (CNTF)对脊髓神经元的保护作用。 方法 :用改良 Allen打击法致 SD大鼠 T8脊髓损伤 ,分别于术前及术后第 3、7、14天测定脊髓组织内乙酰胆碱酯酶 (ACh E)和酸性磷酸酶 (ACP)的含量。 结果 :ACh E于术后持续下降 ;ACP在术后 3天开始上升 ,第 7天升至最高 ,以后逐渐下降 ;经 CNTF治疗后上述两种酶变化的幅度较小 ,且整个过程也比较平稳。 结论 :CNTF对脊髓损伤的神经元具有一定的保护作用。
Objectives:To study the enzymatic changes and protective effects of ciliary neurotrophic factor (CNTF) on neurons in spinal cord injury (SCI). Methods: SD rats were subjected to incomplete SCI with modified Allen methods. Activities of acetylcholinesterase (AChE) and acid phosphatase (ACP) in spinal cord were quantitatively measured preoperatively, and 3, 7, 14 days after SCI. Results:AChE activity decreased gradually after injury. ACP activity increased 3 days after injury, and reached peak on the 7th day, then recovered gradually. The rats treated with CNTF showed relatively smooth change of the 2 kinds of enzymes. Conclusions:CNTF plays a role in protecting the spinal cord neurons against injury.
出处
《医学研究生学报》
CAS
2001年第2期132-134,共3页
Journal of Medical Postgraduates
关键词
脊髓损伤
睫状神经营养因子
神经元
酶
Spinal cord injury
Ciliary neurotrophic factor
Neurons
Enzymes
