黄芪对沙土鼠脑缺血-再灌注损伤的影响

Influences of astragulas(黄芪) on cerebroischemia-reperfusion injury in gerbil

目的 :研究黄芪对沙土鼠脑缺血再灌注损伤的影响。方法 :结扎沙土鼠双侧颈总动脉致脑缺血 15分钟再灌注 48小时 ,造成脑组织迟发型神经元死亡 (DND)模型 ,比较假手术组、模型组和黄芪治疗组鼠脑组织中 Na+ K+ ATP酶活性 ,一氧化氮合酶 (NOS)活性、一氧化氮 (NO)和兴奋性氨基酸含量的变化。结果 :(1)与假手术组比较 ,模型组和黄芪治疗组鼠脑组织中 Na+ K+ ATP酶活性均明显降低 ,但模型组比黄芪治疗组降低更明显 (P均 <0 .0 1)。 (2 )各组鼠脑组织乳酸 (L D)含量变化未见统计学差异。(3)与假手术组比较 ,模型组和黄芪治疗组鼠脑组织 NOS活性和 NO含量均降低 ,但模型组降低更明显 (P<0 .0 5 )。 (4 )与假手术组比较 ,模型组和黄芪治疗组脑组织谷氨酸 (Glu)含量均高于假手术组 ,但模型组的升高更明显 (P<0 .0 5 )。 (5 )模型组和黄芪治疗组脑组织天冬氨酸 (Asp)含量均高于假手术组 (P均 <0 .0 5 )。模型组 Asp含量略高于黄芪治疗组 ,但差异无显著性。 (6 )各组脑组织γ氨基丁酸 (γ GABA )和甘氨酸 (Gly)含量的比较差异无显著性。结论 :黄芪抗脑缺血再灌注损伤的机制可能还与其防止脑缺血后脑组织 Na+ K+ ATP酶活性、NOS活性。

Objective:To study the influences of astragulas(黄芪) on cerebroischemiareperfusion injury in gerbils.Methods:The cerebral tissue delayed neuron death(DND) models of gerbil were established by means of ligating bilateral carotid arteries for 15 minutes then reperfusing for 48 hours and divided into shamoperation(SO) group,ischemiareperfusion(IR) group and astragulas injection (AI,黄芪注射液) group.The activities of Na+K+ATPase and nitric oxide synthase(NOS) and the changes in contents of nitric oxide(NO) and excitatory amino acid(EAA) in cerebral tissue were compared in different groups.Results:(1)In comparison with SO group the activities of Na+K+ATPase in IR and AI group were obviously decreased furthermore the decreases in IR group were more significant than those in AI group(all P<0.01).(2) The changes in contents of lactic acid(LD) in cerebral tissue in all the groups had not statistically significance.(3)In comparison with SO group the activitis of NOS and the contents of NO in IR and AI group were reduced but the reductions in IR group were much more significant (P<0.05).(4)The contents of glutamic acid(Glu) in IR and AI group were higher than those in SO group and the increases in IR group w.05),meanwhile they in IR group were higher than those in AI group but the difference was not significant.(6)Among all the groups the contents of γaminobutyric acid (γGABA) and glycine(Gly) had not significant differences (all P>0.05).Conclusions:The mechanisms of astragulas on anticerebral ischemiareperfusion injury may be related to its preventing the reductions of activities of Na+K+ATPase,NOS,and the decreases in synthetic volume of NO as well as its heightening the contents of EAA after ischemia of brain tissue.