山羊发情周期中及妊娠期中毛孕酮浓度的变化

HAIR PROGESTERONE CONTENTS DURING OESTRUS CYCLE ANDPREGNANCY IN GOATS

本试验用放射免疫分析(RIA)药盒测定了沙能,杂交1代(沙能×成都麻羊)和杂交2代〔沙×(沙×成)〕共23头山羊发情周期中及妊娠期中奶孕酮和毛孕酮浓度的变化。试验结果表明,应用RIA药盒可测出毛申孕酮。配种后1—28天,孕羊奶和毛中孕酮含量,非孕羊奶和毛中孕酮含量均呈极显著相关(分别为r=0.5458,p<0.01和r=0.7832,p<0.01)。配种后22天,以每毫升奶含孕酮3.9ng为判斯孕否标准,孕与非孕羊确诊率分别为82.4％和100％;以每50mg毛含3.7ng孕酮为判断孕否标准,孕与非孕羊确诊率分别为77.8％和100％。妊娠20天起,毛中孕酮含量(5.67±0.98ng/50mg毛)逐渐上升,120天达到高峰(9.85±1.20ng/50mg)。产羔前8天起,毛中孕酮含量(7.73±1.91ng/50mg)下降较快,产羔当天下降到4.93±0.25ng/50mg。

Hair progesterone contents during gestasion and milk progesterone levels during oestrus cycle in Saanen (S),crosses F1(S×Chengdu Mah) and F2 (S×(S×Chengdu Mah) ) goats were determined using the RIA kit.The results showed that progesterone in goats hair could be detected using the RIA kit.In pregnant goats,hair progesterone contents was correlated with the milk progesterone profile during 1-28 days after oestrus (r= 0.5458,P<0.01 ).In non-pregnant goats,similar correlation was observed (r=0.7832,p<0.01).After milk samples were collected 22 days,3.9ng /ml of progeste'rone was taken as the discriminatory level,and precision of pregnancy and non-pregnancy diagnosis were 82.4% and 100% respectively.After hair samples were collected 22 days,3.7ng/50mg of progesterone was taken as discriminatory level,and precision.of pregnancy and non-pregnancy diagnosis were 77.8% and 100% respectively.During gestation,hair progesterone content increased gradually from day 30 (5.67±0.98ng/ 50mg hair) to day 120 (9.85±1.20ng/50mg ) and decreased rapidly from-8 (before parturition,7.73±1.91ng/50mg ) to day 0 ( parturition,4.93±0.25 ng/50mg)