营养因素对日本血吸虫毛蚴人工转变母胞蚴及体外培养的影响

EFFECTS OF NUTRITIVE FACTORS ON SCHISTOSOMA JAPONICUM MIRACIDIAL TRANSFORMATION AND MOTHER SPOROCYST CULTURE IN VITRO

本文报道了4种培养基、4种血清、不同浓度的半乳糖、二巯基苏糖醇及部分三羧酸循环中间产物对日本血吸虫毛蚴体外转变为母胞蚴及其存活的影响。发现稀释一倍的RPMI 1640加10％兔血清为较适宜的培养基。上述添加剂对毛蚴转变和母胞蚴存活均无有益作用,其中柠檬酸可显著抑制毛蚴的转变。文中描述了毛蚴在体外转变成母胞蚴的过程,对血吸虫的糖代谢途径进行了讨论。

This paper observed the effects of several factors on the transformation of miracidia of Schistosoma japonicum and on the culture of young mother sporocysts of S. japonicum in vitro. The results showed: Different media (RPMI 1640, Tc 199, BME, and half strength RPMI 1640) and different serums (calf, sheep, rabbit, and 'O' type human serum) did not affect the transformation. The longest survival seen in half strength RPMI 1640 was 48 days. The development of germinal balls was observed. The length of some parasites was nearly doubled, at 164μm. The serum of rabbit was most beneficial to the survival of the parasites. Galactose and DTT could not promote the transformation and the survival. Citrate, -ketoglutarate, malate and sodium succinate were not helpful to the survival of parasites. They inhibited the transformation and the effect of citrate was significant. The process of miracidial transformation in vitro was discribed and the ways of carbohydrate metabolism in mother sporocysts were discussed. Authors suggested 'Latin square' as an optimum design in studying the composition of medium.